Effectiveness of a phage cocktail as a biocontrol agent against L. monocytogenes biofilms.
Food Control, 2017
Sadekuzzaman, M., S. Yang, M. F. R. Mizan, H.-S. Kim, and S.-D. Ha.
Abstract
Listeria monocytogenes can persist and form biofilms in a food environment which are difficult to eradicate because biofilms are inherently resistant to a variety of antimicrobial treatments. Therefore, alternative approaches such as bacteriophages have been suggested as a promising biocontrol agent against biofilms. The aim of this study was to evaluate the efficacy of a cocktail bacteriophage product (ListShield™) against L. monocytogenes biofilms. These biofilms were established on lettuce, stainless steel, rubber, and a MBEC biofilm device and exposed to the ListShield™ phage preparation (1x108 PFU/ mL) for 2 h. ListShield™ had sufficient potency to significantly reduce the biofilm (P < 0.05) in all cases. Biofilm reduction achieved after ListShield™ treatment on the stainless steel coupon was 1.9e2.4 log CFU/cm2 and on the rubber surface approximately 1.0 log CFU/cm2. Phage application on lettuce inactivated biofilm bacteria up to 0.7 log CFU/cm2. These results suggest that bacteriophage preparation ListShield™ is an effective tool for the inactivation of L. monocytogenes biofilms in the food industry.
Target
Listeria monocytogenes
Food Group
Biofilm
Food Product
Lettuce, Rubber, Stainless Steel
Product
Lettuce, Rubber, Stainless Steel
Catalog Number
01LP