Scientific Literature
 

Reduction of Salmonella on chicken meat and chicken skin by combined or sequential application of lytic bacteriophage with chemical antimicrobials.

Authors
Sukumaran, A.T., Nannapaneni, R., Kiess, A., and Sharma, C.S.

Journal
International Journal of Food Microbiology, 2015

https://doi.org/10.1016/j.ijfoodmicro.2015.04.025

Abstract
The effectiveness of recently approved Salmonella lytic bacteriophage preparation (SalmoFresh™) in reducing Salmonella in vitro and on chicken breast fillets was examined in combination with lauric arginate (LAE) or cetylpyridinium chloride (CPC). In another experiment, a sequential spray application of this bacteriophage (phage) solution on Salmonella inoculated chicken skin after a 20 s dip in chemical antimicrobials (LAE, CPC, peracetic acid, or chlorine) was also examined in reducing Salmonella counts on chicken skin. The application of phage in combination with CPC or LAE reduced S. Typhimurium, S. Heidelberg, and S. Enteritidis up to 5 log units in vitro at 4°C. On chicken breast fillets, phage in combination with CPC or LAE resulted in significant (p < 0.05) reductions of Salmonella ranging from 0.5 to 1.3 log CFU/g as compared to control up to 7 days of refrigerated storage. When phage was applied sequentially with chemical antimicrobials, all the treatments resulted in significant reductions of Salmonella. The application of chlorine (30 ppm) and PAA (400 ppm) followed by phage spray (109 PFU/ml) resulted in highest Salmonella reductions of 1.6 – 1.7 and 2.2 – 2.5 log CFU/cm2, respectively. In conclusion, the surface applications of phage in combination with LAE or CPC significantly reduced Salmonella counts on chicken breast fillets. However, higher reductions in Salmonella counts were achieved on chicken skin by the sequential application of chemical antimicrobials followed by phage spray. The sequential application of chlorine, PAA, and phage can provide additional hurdles to reduce Salmonella on fresh poultry carcasses or cut up parts.

Target
Salmonella spp.

Product
SalmoFresh

Catalog Number
02SP

Food Group
Poultry

Food Product
Chicken skin and meat

Back to publications archive



Technology

Food Safety

Environmental Sanitation

Veterinary Applications

Human Therapeutics

Probiotics